*CUBIC reagents are commercialized under invention licenses by RIKEN, Japan.

CUBIC-L (Product Number: T3740) and either CUBIC-R+(N) (Product Number: T3983) or CUBIC-R+(M) (Product Number: T3741) are required for tissue-clearing.
Please use a combination with CUBIC reagents according to your samples and purposes.

Products

†If you want to know when to use Mounting Solution, please see Q&A.

†Refer to CUBIC-HV™ page for more information on CUBIC-HV™.

Advantages

• BASIC PROTOCOL: Soaking in only two products makes mouse whole-body or animal tissues clear. CUBIC-L (for delipidation and decoloring) and either CUBIC-R+(N) (for RI matching) or CUBIC-R+(M) (for RI matching).
  CUBIC-L: for delipidation and decoloring
  CUBIC-R+(N): for RI matching
  CUBIC-R+(M): for RI matching

• OPTIONAL PROTOCOL: The following products can easily clear tissues which were previously difficult to clear.
  CUBIC-B: for bone
  CUBIC-HL: for highly fatty tissues and quenching autofluorescence
  CUBIC-P: for mouse perfusion efficiently aids with perfusion fixation

• EXPANSION PROTOCOL: The following products can clear tissues with expansion.
  CUBIC-X1: for expansion tissues
  CUBIC-X2: for RI matching with keeping the expanded size

• Preserve the fluorescent protein signals except CUBIC-HL.

• Staining thick and large specimens uniformly
  CUBIC-HV™1 3D immunostaining kit: for 3D immunostaining
  CUBIC-HV™1 3D nuclear staining kit: for 3D nuclear staining

• Shorter sample treatment period.

• Using light-sheet fluorescent microscopy (LSFM) or confocal laser-scanning microscopy (CLSM) enables the whole-organ/body imaging at a cellular resolution.